Result files of the KP4 detection protocole (the output of each step is usually the input of next one):

1) Reference PDB templates for TMalign validation: 2 proteins

2) Structural alignment of 1KPT Foldseek hits with TM-score>0.5: 378 proteins

3) Hmmer hits in Fungi secretomes (length<180, 10 iterations, E-value<1, Overlap>=75%): 972 proteins

4) Structural alignment of ZtKP4 Foldseek hits with TM-score>0.5: 866 proteins

5) Hmmer hits in Fungi secretomes (length<180, 10 iterations, E-value<1, Overlap>=75%): 1307 proteins

6) Representatives of AFDB clusters similar to those detected by Foldseek (E-val<0.001, sequence<180aa and more than 50% fungal cluster members: 173 proteins

7) Representatives of AFDB clusters covering all hits: 324 proteins

8) AFDB cluster representatives shorter than 180 aa and not transmembrane: 221 proteins

9) TMalign structural alignment of AFDB representatives versus 1kptA template: 222 proteins

10) AFDB representatives with TM-score>=0.5 versus 1kptA template: 140 proteins

11) TMalign structural alignment of AFDB representatives versus 0ZKP4A template: 222 proteins

12) AFDB representatives with TM-score>=0.5 versus 0ZKP4A template: 143 proteins

13) Accession numbers of the AFDB representatives validated by any template: 153 proteins

14) Corresponding secreted AFDB50 cluster representatives (sharing less than 50% seq identity): 1138 proteins

15) TM-scores of detected cluster representatives vs 1kptA (columns with >50% gaps removed): 153 proteins

16) AFDB50 representatives alignment with taxonomic information (columns with >50% gaps removed): 153 proteins

17) Phylogenetic tree of ADFB50 representatives: 153 proteins

18) All validated AFDB cluster members: 4381 proteins

19) Taxonomic distribution of all detected KP4 folds: 3722 proteins

20) Most frequent Uniprot annotations: 3722 proteins

21) TMalign alignment of Hmmer hits in Zymoseptoria tritici (vs ../pdb/0ZKP4A.pdb): 5 proteins

22) TM-scores of all Zymoseptoria tritici proteins in final set (vs ../pdb/0ZKP4A.pdb): 29 proteins

23) Same alignment with 2D structure information added: 29 proteins

24) FastME tree of all Zymoseptoria tritici proteins in final set (vs ../pdb/0ZKP4A.pdb): 29 proteins

25) Clustering at 30%id and 60% overlap of KP4 detected by FHAT or Krasileva or Raffaele: 1496 proteins

26) Comparison between protocoles FHAT - Krasileva - Raffaele: 1496 proteins

27) Structural alignment by TMalign of selected proteins vs ../pdb/0ZKP4A.pdb (prefix OK=>detected, No=>not detected): 6 proteins

28) FastME tree derived from structural aligment of selected proteins (OK=>detected, No=>not detected): 6 proteins

29) Representatives from representatives 1D+2D clustering (at 40%id max): 44 proteins

30) Representatives from ZYMTR hits 1D clustering (at 40%id max): 9 proteins

31) Representatives from selected proteins clustering (at 30%id max): 5 proteins

32) FHAT + ZYMTR + MHL representatives in FASTA format: 58 proteins

33) FHAT + ZYMTR + MHL representatives in PDB format (TGZ archive): 58 proteins

34) Uniprot annotations from FHAT+ZYMTR+MHL representatives: 58 proteins

35) FHAT+ZYMTR+MHL representatives structural alignment from TMalign vs ../pdb/0ZKP4A.pdb (without columns with more than 50% gaps): 59 proteins

36) FHAT+ZYMTR+MHL FastME tree derived from TMalign alignment without 50%gap columns: 59 proteins

37) FHAT+ZYMTR+MHL FastME tree derived from TMalign alignment without 50%gap columns with Pfam suffixes: 59 proteins

38) Comparison of FHAT+ZYMTR+MHL trees derived from TM-scores matrix (left) and TMalign structural alignment (right): 59 proteins